Nanolive 3D CX Macrophage Label-Free Real-Time 3D Imaging Assisting Immunology Study

With the Nanolive 3D CX, what is the need for "996" for immunology research?

Macrophages are found in almost all tissues, are immune cells, have multiple functions, and are important targets for studying cell phagocytosis, cellular immunity, and molecular immunology. They contribute to the health of the body's processes such as development, wound healing, infection and intra-tissue balance. They can change their phenotype quickly depending on the environment. Macrophages are known for their classic function as antibacterial phagocytic cells, but also support immune function through antigen expression. Their research applications are very extensive, and in vitro testing is widely used in various research fields, including immunology, bacteriology and parasitology, as well as biomedical and transplantation research. Two advantages of macrophages in cell culture are that they are relatively easy to obtain and culture.

Nanolive human M1 macrophage marker-free real-time 3D imaging

This video shows a video of a cryopreserved human M1 macrophage culture process from PromoCell, taken by Nanolive's 3D Cell Explorer. The 3D Cell Explorer can image live macrophages in a novel, label-free manner, which is especially worthwhile. It is noted that the visual effect of the film wrinkles is such that the slab-like foot front has a wave and moves toward the main cell body. Shooting method: Macrophages were imaged for more than 24 hours at a frequency of 1 image per 10 seconds.

See the link for the video:
https://mp.weixin.qq.com/s__biz=MzIxMTcwNTg2OQ==&tempkey=MTAwNV8rMUc5YjVuckR
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Ksm=17500c592027854f303daca7c3f3ff213f8c3be8e67f7bcc9f0aeb87539ca2609ee8b78558de
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Cell viability and macrophage function (Video 2) were determined using the PromoCell Phagocytosis assay kit. It can be seen that oval E. coli is surrounded, transported and finally cleaved by cells, so 3D Cell Explorer provides a powerful screening system for activators and/or inhibitors of phagocytic and toll-like receptor (tlr) ligands. .

See the link for the video: https://mp.weixin.qq.com/s__biz=MzIxMTcwNTg2OQ==&tempkey=MTAwNV8rMUc5YjVuckR
6eFRCWmpRX0tPT2U0YnU1SmJfNWpJUmxlekFDcUg0d2VMQ2RvdVMyUjdZaUgyX2dVNDNYa
Gpvd0lxNWtSaEQ5cGxxckFHV21Qd0FSaEd0ZkZwMFBQQi1UV0EyTGg2ekREY2lsTGhHdFlwYmg
xTzhUMjNYZFVyeWpicU5BMmc4RnhITXA2NlQzZVlXUTBUbFhqZ09IbXJfTmI4X2xRfn4%3D&ch
Ksm=17500c592027854f303daca7c3f3ff213f8c3be8e67f7bcc9f0aeb87539ca2609ee8b78558de
#rd

Perfect murder - T cell killing macrophages without labeling

The importance of T cells and macrophages in the immune system

As part of the immune system, T cells are able to recognize HLA-presenting proteins, which are not part of normal cells, so HLA-presenting proteins can imply the presence of pathogens in cells, and T-cells circulate in the blood, with proteins on the surface called T-cell receptors. These receptors scan and pair HLA display proteins, the body is equipped with a large number of T cells, each cell presents only one T cell target receptor, and receptor changes in the library can identify a variety of different pathogens. A given T cell receptor is antigen-matched to HLA, which shows that the adaptive immune system begins to act and initiates an immune response.

Macrophages are a class of white blood cells of the immune system that can encapsulate and digest cell debris, foreign matter, microorganisms, tumor cells, and any other somatic cells that are considered unhealthy.

Perfect murder - T cell killing macrophage 3D marker real-time monitoring

In this animation, we observed the interaction between macrophages and T cells, and the naive T cells with antigens were instructed by macrophages to identify which cells (such as cancer cells) killed them during these interactions, T Cells can function to stabilize the immune system (Andersen, 2018). By killing macrophages with antigen present, it has been shown that cells are trapped on the surface of macrophages with specific markers and tell T cells to induce companion macrophages. Apoptosis, dead macrophages are recovered by other macrophages, giving up space for new macrophages while maintaining the same comprehensive macrophage population.

The video below shows the process of pre-stimulated antigen-presenting cells cultured with freshly isolated naive T cells from the spleen of OT-I rats, observed continuously for 16 hours at a frequency of 10 s (APCs, named trees). Dendritic cells, macrophages, isolated from bone marrow cells of C57BL/6 rats, isolated and obtained from C57BL/6 mouse differentiated bone marrow cells, pre-stimulated with IFNγ and LPS, all primary cells are Freshly isolated from rats (C57BL/6), naive T cells were derived from OT-I rats.
See the link for the video:
https://mp.weixin.qq.com/s__biz=MzIxMTcwNTg2OQ==&tempkey=MTAwNV8rMUc5YjVuckR
6eFRCWmpRX0tPT2U0YnU1SmJfNWpJUmxlekFDcUg0d2VMQ2RvdVMyUjdZaUgyX2dVNDNYa
Gpvd0lxNWtSaEQ5cGxxckFHV21Qd0FSaEd0ZkZwMFBQQi1UV0EyTGg2ekREY2lsTGhHdFlwYmg
xTzhUMjNYZFVyeWpicU5BMmc4RnhITXA2NlQzZVlXUTBUbFhqZ09IbXJfTmI4X2xRfn4%3D&ch
Ksm=17500c592027854f303daca7c3f3ff213f8c3be8e67f7bcc9f0aeb87539ca2609ee8b78558de
#rd

The unique advantages of Nanolive 3D CX in immunology research

* Non-invasive or no staining mark;

* Holographic 3D imaging based on cell physical refractive index;

* The experimental treatment is less than 5 minutes, and the cells are not damaged;

*1.7 seconds of fast 3D holographic imaging;

* Any refractive index based digital staining up to 7 colors;

*167nm XY axis ultra high resolution;

* Real-time monitoring of cell characteristics for up to several weeks;

* Integrates 3-channel fluorescence, which can be seamlessly superimposed with 7 digital dyes;