Cyclic RNA research methods - help you quickly publish 5 or more articles

By 2015, Yunxu has completed 10000+ circular RNA full transcriptome sequencing services, covering 50+ diseases and 20+ species, helping many customers to make breakthroughs in the research of cyclic RNA molecules. Customers have published more than 20 articles on circular RNA. In addition, Cloud Sequence Bio provides research tools for circular RNA mechanisms, rip & RNA pull down technology, to help customers sprint high-scoring mechanism articles. This week, Xiaobian talked about how the cloud-order bio-customer used the whole transcriptome sequencing to quickly publish an article with more than 5 points. (In the previous period, I reported a lot of cases in which circular RNA sequencing directly published 5 articles. Interested. Partners can click on the previous link).

Research Background:
As an important member of non-coding molecules, circular RNA molecules play an important role in a variety of disease physiology processes. This time, studies on circular RNA molecules focused on the effects of ionizing radiation on cells. Speaking of ionizing radiation, in fact, in our lives, trace radiation is almost ubiquitous. There are natural sources of radon in the soil, X-ray machines used in physical examination, and so on. It is important to know that intestinal epithelial cells are highly sensitive to the ionizing radiation of intestinal epithelium. Patients with abdominal or pelvic tumors may cause intestinal damage caused by radiotoxicity after receiving radiotherapy. Then the question comes, how does the intestinal damage caused by radiation compare with the normal intestinal cells, and how does the circular RNA molecule change this time? What is the function of the different cyclic molecules? And listen to Xiaobian slowly explain this article for you.

experimental design:
The entire experiment was designed as a control group and an irradiation treatment group (experimental group), with three biological replicates in each group. The control group was normal wild-type mice, and the experimental group was irradiated with a constant meter using a Cs137 gamma light radiometer. After 3.5 days, RNA was extracted from the jejunum tissues of two groups of mice.

Figure 1. Technology roadmap

research content:
1. What is the expression of circular RNA? What are the differences in expression?
High-quality sequencing services, professional biometric analysis, cloud-order biology make ring research easy, this is an advertisement. High-quality sequencing technology accurately detects the expression of each loop. The biometric analysis visualization gives all the circular RNA in the jejunum of mice after radiotherapy from a macroscopic perspective (chromosomal distribution, loop position). The expression of molecules.

Figure 2. Expression of circular RNA in jejunum tissue of mice after radiation treatment

On this basis, which circular RNA molecules are differentially expressed? Here, the volcano map and the Venn map are all available. According to the criteria of p<=0.05 and FC>=2, a total of 42 loops were found to be up-regulated and 47 loops were down-regulated.

Figure 3. Screening of differential circular RNA

2. qPCR verification of differential circular RNA molecules
For an article on the spectrum, the results of the differential circular RNA are verified by qPCR. Low-throughput qPCR results for 5 up-regulated and 5 down-regulated circular RNA molecules were consistent with high-throughput sequencing results.

Figure 4. qPCR validation of differential RNA molecules

3. Functional prediction of differentially expressed circular RNA molecules
GO analysis and Pathway analysis are also included in this study as an essential part of functional prediction, including enrichment of GO entries such as binding, catalysis, cytokines and cell secretion. KEGG analysis predicted that the target gene of differential circular RNA was associated with MAPK signaling pathway (Cacna1e, Mras, Prkcb, Ptpn7), and recent reports indicate that EGF-mediated activation of Ras/Raf/Mek/Erk/MAPK signaling pathway Intestinal stem cell proliferation is associated.

Figure 5. Functional prediction of differentially expressed circular RNA molecules

The sponge mechanism predicts the ceRNA network map. According to the transregulation of the sponge mechanism, the network includes 42 up-regulated and 48 down-regulated circular RNA molecules, as well as predicted 22 target gene mRNAs that bind to each other.

Figure 6. ceRNA network diagram

to sum up:
Through the analysis of the article, we can see that this is a typical circular RNA molecule. The results shown in the article are similar to most of the published articles. From expression, difference, enrichment to target gene prediction, from beginning to end, But there is a reason for such an article to be published in a 5.5-point magazine. First of all, this study is the first study of the changes of cyclic RNA molecules in intestinal cells under radiation conditions. The treatment and problem solving angles are different. The secondary letter analysis is comprehensive and accurate, and multiple intestinal cells are included in the enrichment analysis. Proliferation, signaling pathways during stress, and gene correlation suggest that loops play an important role in the entire process of cellular biology. Such an affordable and clear-cut article is believed to be a model for doing circular RNA molecules. If you want to publish such an article in a short time, please contact Shanghai Yunxu Bio, we will give you the best scientific research service. .

Full text information:
Qianying Lu, Wei Gong, et al. Identification of Circular RNAs Altered in Mouse Jejuna After Radiation. Cell Physiol Biochem (2018), http://sci-hub.tw/10.1159/000491652

Cloud order related product recommendation:
Whole transcriptome sequencing
Circular RNA sequencing
lncRNA sequencing
mRNA sequencing
miRNA sequencing

Past review:
叕 one! Cloud order customer 12 points top article, teach you how to use RIP sequencing to play the molecular mechanism!
Cloud order customers published the first lupus erythematosus! Teach you how to quickly play the cell issue!
Cloud order customers publish the world's first zebrafish circular RNA research results !
Yunxu customers published the world's first wheat circular RNA research results !
Yun Xuan customers published the first brain trauma exocrine ring RNA article! Teach you how to quickly play 5 points article!
The hotspot of natural research in 2018: a deep analysis of circular RNA research

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