How does the Ohaus ST5000i benchtop ion meter detect milk calcium content?

    Ion meters, also known as ion mobility meters, are used in conjunction with ion-selective electrodes to directly measure liquid samples without the need to pre-treat the sample, resulting in less waste than titration or other methods. Easy to use, easy to operate, and more competitively priced . More and more fields are being used, and many ion meters are widely used in agriculture/soil, clinical analysis, environmental protection, space exploration, life sciences, food and drug analysis.

Let's talk about the determination of the calcium ion concentration that is often used in the food industry. Calcium is an extremely important element in the human body, and milk is a good calcium supplement. Its calcium content is also one of the important indicators for dairy manufacturers.

At present, calcium is usually measured by potassium permanganate titration, EDTA titration, atomic absorption or flame photometry, and the like. However, these methods require ashing the sample, dissolving it with acid, and then measuring it, which is complicated and slow. For perishable milk samples, it is difficult to use for routine analysis. If the calcium in the milk is measured by the ion selective electrode, it is not necessary to be ashed, and the milk sample can be measured after dilution, and the method is simple and rapid . [Note 1]

Yes, Ohaus STISE28 composite calcium ion electrode can accurately measure the concentration of calcium ions in milk. The following small series teaches you how to use calcium ion selective electrode to measure the calcium content in milk. Don't blink your eyes.

Main reagents and instruments

ST5000i ion meter, STISE28 composite calcium ion electrode, magnetic stirrer, pipette, volumetric flask, measuring cylinder, beaker, distilled or deionized water, calcium ion standard solution and ISA solution.

Experimental step

1) Configure the standard solution

The standard solution configuration is recommended to use a stepwise dilution method. Stepwise dilution refers to diluting the initial high concentration of the standard solution with a volumetric flask to obtain a second standard solution; diluting the second concentration standard solution to prepare a third standard solution; and so on, until the required standard is obtained. liquid.

The above-prepared calcium ion standard solution was prepared by pouring 50 ml + 1 ml of ISA (ion strength adjuster) into a 250 ml beaker, and mixing was uniformly performed.

2) Preparing samples

Weigh 50ml of 5 common milk samples on the market, and get 5 milk samples, respectively, +1ml ISA into a 250ml beaker, mix and evenly test.

3) Instrument settings

ST5000i display

4) Standard calibration

5) Milk sample measurement

STISE28 Ca2+ ion electrode

After the prepared milk sample was allowed to stand for five minutes, the concentration of free calcium was measured, and the results are shown in the following table.

Main reagents and instruments

From the above experiments, we obtained that the calcium ion concentration in the sample milk was 143 mg/L.

Test picture of milk sample 5

Excellent performance, extraordinary experience

The Ohaus ST5000i laboratory ion meter combines simple, fast and accurate integration to detect different ion contents without chemical separation. Is such a simple and easy to use ion meter that you are looking for? If you want to know more about its parameters and features, look at the product card below, so that you can see at a glance!

references:

* [1]   : Liu Jianli, Niu Strike, Zhang Hongwei. Chemical Sensors , 1987(3): 85-8 7

The Ohaus Starter family of products is rich in benchtop acidity meters that measure the film's test solution. It also has a benchtop ion meter that measures the calcium concentration in milk. More product knowledge you can't imagine. In the US Ohaus WeChat you are concerned about. If you want to know more about the Starter series, or are looking for more professional and detailed selection guide, please call the hotline and leave relevant information, our professional engineers will contact you in the first time!

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