Exosomes are extracellular small vesicles of a circular monolayer structure with a diameter of 30-100 nm formed by a series of regulatory processes such as "endocytosis-fusion-efflux". Exosomes are released by many types of cells, and are widely distributed in saliva, plasma, milk, urine and other body fluids. They can carry proteins, transport RNA, and play an important role in intercellular substance and information transduction. Exosomes may affect tumor progression by regulating immune function, promoting tumor angiogenesis and tumor metastasis, and directly acting on tumor cells.
Due to the special structure and function of exosomes, it has potential application value. On the one hand, it can be used as a biological indicator for diagnosing various diseases. On the other hand, it can also be used as a therapeutic means. In the future, it may be used as a natural carrier of drugs for clinical use. treatment. Separation and purification of exosomes has always been a concern of researchers, and obtaining high-purity exosomes is crucial for subsequent research.
Figure 1: Schematic diagram of the exosomes
The super-dissociation method is the most commonly used exosome purification method, which is carried out by low-speed centrifugation and high-speed centrifugation, and can separate vesicle particles of similar size. The super-dissociation method is popular because of the simple operation, and the number of vesicles obtained is large, but the process is relatively time-consuming, the recovery rate is unstable, and the purity is also questioned; in addition, the repeated centrifugation operation may cause damage to the vesicles, thereby Reduce its quality.
How to extract exosomes simply and effectively?
Biovision, the global leader in life sciences, gives a professional answer. For different sample source samples and different separation method principles, Biovision offers two types of extraction reagents:
· ExoPureTM Reagent:
ExoPureTM Reagent is based on the principle of chemical precipitation, which allows rapid separation and extraction of microsomes in a single step and is suitable for biological fluids and cell culture media.
| Product name and description | Brand | Item number | product manual |
| ExoPureTM Reagent (Overall Exosome Isolation, biological fluids) | Biovision | M1001 | Details |
| ExoPureTM Reagent (Overall Exosome Isolation, cell media) | Biovision | M1002 | Details |
| ExoPureTM Reagent (Overall Exosome Isolation, urine) | Biovision | M1003 | Details |
Figure 2: ExoPureTM Reagent Lab Showcase
· ExoPureTM Immunobeads:
ExoPureTM Immunobeads covalently coats Exosomes surface antigen-specific antibodies on latex Beads and uses immunological binding to separate high-purity Exosomes in biological fluids and cell culture media.
| Product name and description | Brand | Item number | product manual |
| ExoPureTM Immunobeads (cell media) | Biovision | M1031 | Details |
| ExoPureTM Immunobeads (plasma, urine, serum) | Biovision | M1039 | Details |
| MouseExoPureTM Immunobeads (mouse biofluids) | Biovision | M1035 | Details |
| MouseExoPureTM Immunobeads (mouse cell media) | Biovision | M1037 | Details |
| TumorExoPureTM Immunobeads (Tumor-derived Exosome, biofluids) | Biovision | M1033 | Details |
Figure 3: ExoPureTM Immunobeads Protocol Showcase
As a global life sciences company leader, BioVision has an outstanding influence in the development and research of apoptosis and metabolism. It includes a wide range of products including test kits, antibodies, recombinant proteins and enzymes, as well as other innovative research tools. In addition to traditional areas of apoptosis and metabolic analysis, BioVision's high-quality products also cover cell proliferation, cell stress, cell damage and repair, diabetes, obesity and metabolic syndrome, stem cell biology, gene regulation, signal transduction, etc. Multiple fields of cell biology. BioVision products are currently being sold to more than 60 countries around the world. As a regional distributor of BioVision in China, Agilent will work with BioVision to provide researchers with the best and latest cell biology research tools to continuously expand the quality of their products and services. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.
Raw materials for animal medicine
Conventional veterinary drug raw materials are: oxytetracycline hydrochloride, doxycycline, chlortetracycline hydrochloride, lincomycin sulfate, gentamycin sulfate, tylosin tartrate, florfenicol, water-soluble amoxicillin, sulfamethoxazole, enno Floxacin lactate, ciprofloxacin hydrochloride, ciprofloxacin hydrochloride, metronidazole, ribavirin, vitamin C, astragalus polysaccharide, penicillin potassium.
The traditional Chinese medicine extracts in veterinary medicines are derived from natural Chinese herbal medicines.
It is purified, concentrated and purified in a different ratio from the original drug. It not only retains the natural state and biological activity of the original drug composition structure, but also retains the harmless and
The beneficial exogenous substance essence realizes the micro-quantification and standardization of Chinese herbal medicine raw materials, and provides a new way for the breeding industry to realize green production. The following herbal extracts for veterinary use: Anti-pathogenic microorganisms Chinese medicine: Anything that can inhibit or kill the reproduction of pathogenic microorganisms and improve the immunity of the body is called anti-pathogenic microorganisms
Chinese herbal medicine. According to the theory of traditional Chinese medicine, it can be divided into heat-clearing medicines, exopathic medicines, diarrhea and water-repelling medicines, damp-damping and diuretic medicines, phlegm-relieving and cough-relieving medicines, qi-regulating and Xiao-shi pharmacological blood medicines, and phlegm-relieving and anti-asthmatic medicines.
Raw materials for animal medicine,Nicarbazin in Feeds,Nosiheptide Veterinary,Nosiheptide Antibacterial,Human Gastric Mucin
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