Comparison of price, cost and performance of major sequencers in the market

November 07, 2023

Comparison of performance and price of major sequencers in the market

Instrument: HiSeq X
- Instrument Price ( USD ): $ 1,000,000/ set (must buy from 10 units)
- Length measurement: 2*150
- Maximum data yield at one time: 1.6~1.8 T ( two flowcells)
- Time to run once: 3 days
- Data production for one year of operation: 219T
- Reagent fee per G data ( USD ): $7
- Average base interpretation error rate: 0.2%
- Detailed technical performance: http://mp.weixin.qq.com/s?__biz=MzA4NzE0MTYwOQ==&mid=10013823&idx=1&sn=c93684980ea4c689d9906cc0e19c3f7c#rd
- Official information link: http://res.illumina.com/documents/products/datasheets/datasheet-hiseq-x-ten.pdf

Instrument: HiSeq 2500 Upgrade
- Instrument price ( USD ): 740,000
- Length measurement: 125*2, high-throughput mode; 250*2, fast mode
- Maximum data output at one time: 0.9~1 T (2*125 mode, two flowcells)
- Time to run once: 6 days
- Data production for one year of operation: 60T
- Reagent fee per G data ( USD ): $30
- Average base interpretation error rate: 0.1%
- Factory date limit: HiSeq can be upgraded after 2013, and cannot be upgraded before.
- Official technical information: http://res.illumina.com/documents/products/datasheets/datasheet_hiseq2500.pdf
- video:

Instrument: HiSeq 2500 not upgraded version
- Instrument price ( USD ): 740,000
- Length measurement: 2*150
- Maximum data output at one time: 180G/run (fast mode 2*150, 2 Flowcell; the output of high-throughput mode is the same as HiSeq 2000, see next box)
- Time to run once: 40 hours
- Data production for one year of operation: 32T
- Reagent fee per G data ( USD ): $ 55
- Average base interpretation error rate: 0.260%

Instrument: HiSeq 2000
- Instrument price ( USD ): 654,000
- Length measurement: 2*100
- Maximum data production at a time: 600G/run (2*100 mode, two flowcells)
- Time to run once: 11 days (2*100 mode, two flowcells)
- Data production for one year of operation: 20T
- Reagent fee per G data ( USD ): $41
- Average base interpretation error rate: 0.260%

Instrument: NextSeq 500
- Instrument price ( USD ): 250,000
- Length measurement: 2*150
- Maximum data production at a time: 100~120 G/run (one Flowcell)
- Run time: 29 hours
- Data production for one year of operation: 29T
- Reagent fee per G data ( USD ): $41
- Average base interpretation error rate: 0.2%
- Official technical information: http://res.illumina.com/documents/products/datasheets/datasheet-nextseq-500.pdf
- video:

Instrument: MiSeq
- Instrument price ( USD ): 99,000
- Length measurement: 2*300
- Maximum data production at a time: 13.2~15G ( one Flowcell)
- Time to run once: 65 hours (300*2 mode)
- Data production for one year of operation: 1.6T
- Reagent fee per G data ( USD ): $86
- Average base interpretation error rate: 0.200%
- Official technical information: http://res.illumina.com/documents/products/datasheets/datasheet_miseq.pdf
- Official video:

Instrument: Proton with PI chip ( Proton current status)
- Instrument price ( USD ): 243,000
- Length measurement: 200
- Maximum data production at a time: 10G
- Run time: 2~4 hours
- Data production for one year of operation: 11T (three runs per day)
- Reagent fee per G data ( USD ): $80
- Average base interpretation error rate: 1%
- Official video:

Instrument: Proton with PII chip (future state, maybe PII chip can be launched in 2014 )
- Instrument price ( USD ): 243,000
- Length measurement:?
- Maximum data production at a time: 30G
- Time to run once:?
- Data production for one year of operation: 33T (three runs per day)
- Reagent fee per G data ( USD ):?
- Average base interpretation error rate:?

Instrument: Ion Torrent PGM
- Instrument price ( USD ): 80,000
- Length measurement: 400
- Maximum data output at one time: 1~2G
- Run time: 7.3 hours (400bp), 4.4 hours (200bp)
- Operating data production year: 1.4T (a day to run three run)
- Reagent fee per G data ( USD ): $ 450 (based on 318 chips and 400bp read length)
- Average base interpretation error rate: 1.00%

instrument: Â PacBio RS
- Instrument price ( USD ): 965,000
- Length measurement: 10000bp
- Maximum data production at a time: 0.1G/run
- Time of operation: 2 hrs
- Data production for one year of operation: 146G (running 4 runs per day)
- Reagent fee per G data ( USD ): Â $2000
- Average base interpretation error rate: 12%
- Official video:

instrument: Â 454 GS Junior (the instrument has been discontinued )
- Instrument price ( USD ): 108,000
- Length measurement: 400
- Maximum data yield at one time: 0.035G
- Time of operation: 8 hrs
- Data production for one year of operation: Â 12G
- Reagent fee per G data ( USD ): Â $31000
- Average base interpretation error rate: homopoleer greater than 6 bases is not allowed
- Official video:

instrument: Â 454 FLX (the instrument has been discontinued )
- Instrument price ( USD ): 500,000
- Length measurement: 1000
- Maximum data production at a time: 0.5G
- Run time: 7.5 hrs
- Data production for one year of operation: Â 180G
- Reagent fee per G data ( USD ): Â $16400
- Average base interpretation error rate: homopoleer greater than 6 bases is not allowed

instrument: Â Nanopore
- Instrument Price ( USD ): 1000
- Length measurement: ?
- One maximum data yield: ?
- Time to run once: Â ?
- Data production for one year of operation: Â ?
- Reagent fee per G data ( USD ): ?
- Average base interpretation error rate: ?
- Official video:

instrument: Â ABI 3730XL Â Classic generation sequencer ( 96 channels of capillary)
- Instrument price ( USD ): 357,000
- Length measurement: 900
- Maximum data production at a time: 81,600Bp
- Run time: 2 hours
- Data production for one year of operation: 300M
- Reagent cost per reaction of (USD): USD 1.0 / 900Bp
- Average base interpretation error rate: 0.001%

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Duo Limb Breathing Circuit

The Duo limb circuit is non-rigid tube used to convey gases or vapors between components of a breathing system. This anaesthesia circuit mainly made of EVA material, extremely flexible, kinking resistant, very high quality. Gas sampling line can be attached outside of circuit. The breathing circuit-duo-limb circuit weighs less than two-limb circuits, reduces torque on the patient`s airway. And it`s one single limb only , offers more versatility in use and transportation. Besides, the circuit can be assembled with water trap, anaesthesia mask, Breathing Bag, BV filter, Breathing filters-hmef and so on, to fit with various needs. Thus, there are different models according to the assembled accessories and tube length.

1. Single use, ISO13485, fda approved
2. EO sterilization is optional;
3. Individual PE bag or paper-poly pouch is optional;
4. Standard connector ( 15mm, 22mm );
5. Made mainly of EVA material, very flexible, kinking resistant, very high quality, gas sampling line can be attached outside of circuit;
6. Weighs less than two-limb circuits, reduces torque on the patient`s airway;
7. With one single limb, offers more versatility in use and transportation;
8. The length can be customized variously: 1.2m/1.5m/1.8m/2.4m/2.7m etc.;
9. The breathing circuit can be equipped with Breathing Bag (latex or latex free), Filter, HMEF, Catheter Mount, Anesthesia Mask or Extra Tube etc.
Duo Limb Breathing Circuit,Anesthesia Ventilator Circuit,Single Limb Breathing Circuit,Adult Ventilator Circuit
Hangzhou Trifanz Medical Device Co., Ltd , https://www.cfzmeds.com

Comparison of price, cost and performance of all sequencers