Competent topic: Cell transient transfection technology
principle: The target gene is introduced into the cell by gene transfection technology such as liposome-mediated transfection and electroporation, and the target gene can be expressed in the cell generally 48 hours after transfection. According to different experimental purposes, experiments such as detection of target gene expression can be performed after 48 hours. application: Observe the function of the gene of interest and its expressed protein in a certain cell (observed in a short time, but the effect will be quickly lost). General process: 1) Cell seeding: Cells were inoculated the day before the transfection experiment, and the plate density of various cells was determined according to the growth rate of various cells and the shape of the cells. The cell density should be 60%~80% coverage on the day of transfection. 2) Cell transfection (liposome, electroporation, FuGENE6, etc.) 3) Identification of the expression of the target gene after 48 hours. Preparation: l Construct a good foreign gene vector, or basic information of the target gene. l Cell lines to be transfected (1 x 106 cells, passageable, doubling time less than 48 hours) and instructions for cell culture conditions. l If you need to detect the expression of the target gene, please provide the corresponding antibody. The results are presented: Constructed transient transfected cells and related exogenous gene expression analysis. Gynecology And Obstetrics Surgical Pack Delivery Obstetric Drape Pack,Natural Labour Delivery Pack,Surgical Gynecology Pack,Birth Packs Universal Surgery Pack Suzhou JaneE Medical Technology Co., Ltd. , https://www.janeemedical.com