The basic principle and operation steps of human α1 acid glycoprotein (α1-AGP) chemiluminescence immunoassay kit
Background introduction Chemiluminescence immunoassay (CHAIA) combines highly sensitive chemiluminescence assays with highly specific immunoassays for various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins and drugs. And other detection and analysis techniques. It is a new immunoassay technology developed after radioimmunoassay and enzyme-free analysis. The CLIA KIT produced by Elabscience uses HRP as a catalyst. Luminol is used as a luminescent substrate and uses Luminol/Enhancer technology developed by the company to make our products sensitive and long-lasting. Under the catalysis of HRP , the peroxide reacts with Luminol/Enhancer to emit an optical signal, and the optical signal is read using a chemiluminescence analyzer. product description Fundamental This kit uses a double antibody sandwich method. The anti-Human α1-AGP antibody was coated on the plate, and the α1-AGP in the specimen or standard was bound to the coated antibody, and the free component was washed away. Biotinylated anti-Human α1-AGP antibody and horseradish peroxidase-labeled avidin were sequentially added. The anti-Human α1-AGP antibody binds to Human α1-AGP bound to the coated antibody, biotin binds to avidin to form an immune complex, and the free component is washed away. The luminescent substrate mixture was added, the luminescent substrate was fluoresced under the catalysis of horseradish peroxidase, and the chemiluminescence value (CL) was measured by a chemiluminescence immunoassay analyzer, and the α1-AGP concentration was positively correlated with the chemiluminescence value. The concentration of α1-AGP in the specimen was determined by plotting a standard curve. Steps 1. Add 100 μL of standard or sample to each well and incubate at 37 ° C for 90 minutes. 2. Pour the liquid in the well, pat dry, add 100 μL of biotinylated antibody working solution, incubate at 37 ° C for 60 minutes. 3. Wash 3 times 4. Add 100 μL of enzyme conjugate working solution and incubate at 37 ° C for 30 minutes. 5. Wash 5 times 6. Add 100 μL of substrate solution and incubate for 5 minutes at 37 °C 7. Readings 8. Calculation of results Crab Crab,Dungeness Crab,Blue Crab,Yummy Crab ZHEJIANG EVERNEW SEAFOOD CO.,LTD , https://www.evernewseafood.com  English name  Human α1-AGP (Alpha-1-Acid Glycoprotein) CLIA Kit  Chinese name   Human α1 acid glycoprotein (α1-AGP) chemiluminescence immunoassay kit   Item number  E-CL-H0001c  species  Human / person  specification  96T/Kit (8*12 strips) 48T/Kit (8*6 strips)  Detection method   Double antibody sandwich method   examination range  3.125~200ng/mL  Sensitivity  1.875ng/mL