Identification of fenugreek
Identification (1) Cross-section of the product: 1 epidermis cell, thicker outer wall and upper part of the lateral wall, fine longitudinal sulcus, larger lower cavity, bright zone located 1/3 of the outer side of the cell, and outer stratum corneum. Support cells 1 column, slightly dumbbell-shaped, slightly narrower at the upper end, wide at the lower end, with a strip-like texture on the peristaltic wall. The endosperm cells are mainly mucinous cells with a very thick wall. Cotyledon cells are ambiguous with powdered particles and fatty oil droplets. (2) Take this product powder 5g, add petroleum ether (60 ~ 90 °C) 30ml, heat reflux for 1 hour, filtered, the filtrate was discarded, dregs evaporated, plus 30ml of ethanol, heated to reflux for 1 hour, filtered, The filtrate was evaporated to dryness and the residue was dissolved in 2 ml of methanol to prepare a test solution. Another reference substance of fenugreekine was added and methanol was added to prepare a solution containing 1 mg per 1 ml as a reference solution. According to the TLC (Appendix VI B) test, 6 μl of each of the above two solutions was pipetted on the same silica gel G plate, and ethanol-acetone-hydrochloric acid (10:6:1) was used as a developing agent. Expand, remove, dry, and spray with a dilute potassium iodide test solution - ferric chloride test solution (2:1) mixed solution. In the chromatogram of the test sample, a spot of the same color is shown in [4] at the position corresponding to the chromatogram of the reference substance. Desk Mini Fans,Portable Mini Fan,Mini Fan USB,Mini Fan Cooler,Handheld Mini fan Ningbo Shuangtuo International Trade Co., Ltd. , https://www.nbst-sports.com