The working principle and classification of the centrifuge
First, the working principle of the centrifuge Second, the classification of centrifuges: Â Freeze Dried Vegetable,Freeze Fresh Runner Beans,Freeze Peach Crisp,Freeze Dried Carrot Flakes Ningbo Nutrition Food Technology Co.,ltd. , https://www.collagenworkshop.com
When the suspension containing fine particles is left stationary, the suspended particles gradually sink due to the action of the gravitational field. The heavier the particles, the faster the sinking, whereas the particles with a lower density than the liquid will float. The rate at which particles move under the gravitational field is related to the size, morphology, and density of the particles, and is related to the strength of the gravitational field and the viscosity of the liquid.
In addition, the substance is accompanied by a diffusion phenomenon when it settles in the medium. Diffusion is unconditional and absolute. Diffusion is inversely proportional to the mass of the material, and the smaller the particle, the more severe the diffusion. The settlement is relative, and if it is conditional, it must be subjected to external forces to move. The settlement is proportional to the weight of the object, and the larger the particle, the faster the sedimentation. For particles smaller than a few micrometers such as viruses or proteins, they are in a colloidal or semi-colloidal state in solution, and it is impossible to observe the sedimentation process by gravity alone. The smaller the particles, the slower the sedimentation, and the more severe the diffusion phenomenon. Therefore, it is necessary to use a centrifugal machine to generate a strong centrifugal force in order to force these particles to overcome the diffusion to produce a settling motion. Â
Centrifugation is the use of a powerful centrifugal force generated by the high-speed rotation of the centrifuge rotor to accelerate the sedimentation velocity of the particles in the liquid, separating the sedimentation coefficients and buoyancy-density materials in the sample.
There are three classification methods for centrifuges: by purpose, by speed, and by structure. Â
â—Classify by use: It is divided into preparative centrifuge and preparation analytical centrifuge. Â
Preparative centrifuge: A centrifuge that only separates and concentrates and purifies the sample. Â
Preparation and Analysis Centrifuge: It can not only separate and concentrate the sample, but also can observe, take photos, measure, digital output and print the centrifuge of the sample through the optical system. Â
â— Classification by speed: divided into low speed centrifuge, high speed centrifuge, ultracentrifuge. Â
Low speed centrifuge: The speed is within 10000 rpm or the relative centrifugal force is within 15000 × g. Â
High-speed centrifuge: The rotation speed is within 10000∽30000rpm or the relative centrifugal force is within 15000∽70000×g. Â
Ultracentrifuge: The rotation speed is above 30,000 rpm or the relative centrifugal force is above 70,000 × g.
â— Classified by structure: Â
Generally, high-speed and low-speed centrifuges are classified according to structure and function, and there are many varieties, and there is no uniform standard for each manufacturer. Can be divided into desktop centrifuge, multi-tube micro-tabletop centrifuge, cell smear centrifuge, blood washing desktop centrifuge, high-speed refrigerated centrifuge, large-capacity low-speed refrigerated centrifuge, low-speed refrigerated centrifuge, desktop high-speed refrigerated centrifuge, desktop Low-speed automatic balancing centrifuges, etc. In addition, there is a triple-type (five-type) high-speed refrigerated centrifuge in foreign countries, which is specially used for continuous centrifugation.
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