Experimental method for liquid nitrogen grinding of corn leaves using TL2020 automatic freezing grinder
purpose: Mass milling of corn leaves provides convenience for subsequent extraction of nucleic acids for genetic or physiological biochemical analysis. For the seed and agricultural product quality management department, seed companies and other units to use molecular methods to detect sample purity, detect the authenticity of purity and other sample pre-processing work necessary for reference. Location: A certain industry breeding and cultivation laboratory Material: fresh corn seedling leaves Instrument: Main unit, TL2020 automatic freeze grinder (also known as TL2020 high-flux tissue grinder); Accessories: 2ml grinding adapter , 3 or 5mm diameter grinding beads Figure 1 : TL2020 Automatic Freeze Mill step: 1 ) Sampling: Take the leaves of fresh corn seedlings and cut them into pieces of 6~8mm in diameter; 2 ) Place 3-5 leaf fragments in a 2 ml centrifuge tube ( EP tube) while adding 1 5 mm and 2 3 mm stainless steel ground steel balls. 3 ) Seal the EP tube cap with the blade and the grinding steel ball into the 2ml grinding adapter of the TL2020 automatic freezing mill , then immerse it in liquid nitrogen with the adapter for 2-3 minutes or until the liquid nitrogen does not “boilingâ€. until. 4 ) Using the DHS special liquid nitrogen grinding operation basket to quickly remove the adapter from the liquid nitrogen and quickly fix it in the rocker arm of the grinder; 5 ) Set the grinding time of TL2020 to 2 minutes and the grinding frequency to 1500 rpm ( 1500 times / min). result: Very finely divided leaf powder is obtained, and subsequent extraction of nucleic acids works well. in conclusion: Using the TL2020 automatic freeze grinder for liquid nitrogen grinding of corn leaves, you can directly immerse the grinding tube and adapter in liquid nitrogen, and only need one-time liquid nitrogen freezing for 1-2 minutes and grinding for about 2 minutes. Up to 56 samples of 2.0 ml were pulverized in batches ( 192 samples with 96- well deep well plates). The treatment time is fast, effectively preventing the temperature rise, and providing a guarantee for the subsequent effective extraction of nucleic acids. In addition, it also saves a lot of liquid nitrogen and is safer to operate. Sweetener Aspartame,Sweetener For Food,Food Ingredients Aspartame,Sweeteners In Food Additives CHANGZHOU GUANGHUI FOOD INGREDIENTS CO.,LTD , https://www.gsweetfood.com
