[Nature Supplement] HoloMonitor M4 Anti-angiogenesis Therapy for Cancer Research
Foreword Although anti-angiogenic (AA) therapy has been widely used in clinical cancer therapy, there are still two major problems of insufficient efficacy and intrinsic resistance. Some studies have shown that anti-angiogenic therapy may even be possible. Increase the likelihood of tumor metastasis. However, in particular, this tumor metastasis caused by anti-angiogenic drugs is still a certain "defect" in the treatment itself. In this study, we used physical barriers to completely block the blood supply to the tumor and observe changes in the tumor's movement. Experimental result The pre-study experiments used physical barriers to block blood supply. The results showed that the tumor cells that were cut off from the blood supply were not affected, but new tumor tissues were formed around them. Further, the primary tumor cells were found to undergo shape mutation. Blocking the physical barrier of blood supply, thereby invading peripheral tissues to form new tumor tissue. Compared with the experimental results of the tumor cells that did not block the blood supply, the above experimental results showed that there was no significant difference in mortality between the two experimental mice, that is, the short-term anti-angiogenic treatment program could not be effectively treated. Tumors, on the other hand, may cause even worse results. Secondary tumors induced by the peripheral area of ​​the primary tumor may carry some invasive ability or resistance to drugs resistant to anti-angiogenesis. In order to determine whether it will affect the migration of the tumor, physical blockage is separately collected. After the tumor tissue escapes cells, does not block the tumor tissue migration cells, and the primary tumor cells, respectively, called Escaped cells, Migrated cells, Primary cells, using the Swedish laser holographic living cell imaging and analysis system ( HoloMonitor M4 ) The monitoring was carried out for 24 hours, and the imaging was performed once every 4 minutes. The migration distance and the movement rate of 19 cells were analyzed by Hstudio 2.6.1 . The results showed that the overall mobility of Escaped cells and Migrated cells was significantly greater than that of Primary cells, and the mobility of Escaped cells was greater than that of Escaped cells. Migrated cells, Transwell results, verify this result. It is shown that the invasiveness of cells escaping from migration from blocking tumor tissue is significantly increased. Therefore, AA treatment creates an extreme environment of nutrient and oxygen deficiency, but does not prevent cancer cell migration, which causes tumor cells to be forced to escape from the "starvation" area and invade other locations. At this time, there is no doubt that the primary tumor Tissues shrink due to insufficient blood supply, but escaped cells induce secondary tumors and even spread throughout the body. Therefore, even if the invasiveness of the tumor does not increase, the "blood deficiency" itself can cause tumor metastasis. Most of the patients who cause death are not primary tumors but metastatic tumors, so where are the benefits of AA treatment? The “inherent vice†in the anti-angiogenic theory may cause the highly metastatic cancer to spread more aggressively Zhang Wei, Center for Molecular Medicine, West China Hospital, Sichuan University: Roasted Squid Snacks,Argentine Squid Sticks,Illex Squid Zhejiang ocean family co.,ltd , https://www.ocean-family.com