Introduction of the principle kit for human neutrophil defensin (HNP1-3)
Human neutrophil defensins (alpha-defensins, HNP1-3) are an important part of the body's natural defense system. HNP1-3 has extensive bactericidal activity, cytotoxicity, immune regulation and chemotaxis, and is also involved in host defense and inflammatory responses. Activated neutrophils release the defensin rapidly. Many studies have shown that in normal plasma, the content of HNP1-3 is very low, about 50-100ng/ml, which is basically undetectable; when the body presents an infectious disease, the content of HNP1-3 can reach 10mg/ml or higher. . Therefore, detecting HNP levels in plasma helps to assess the severity of the inflammatory response for early detection of the disease. At the same time, as a promising antibacterial peptide, HNP has been a hot spot in scientific research. Figure 1: Defensin topology In recent years, the scope of HNP research has gradually expanded. The latest study published in Ped Obesity 2016 (α-Defensins and bacterial/permeability-increasing protein as new markers of childhood obesity) found that HNP can be a classic for children's body mass index and abdominal fat deposition. Mark. More uses of HNP are waiting for researchers to discover, and detection of HNP content is the first step in research! As a regional agent for Hycult Biotech in China, Amy Jie recommends the HyNP brand human HNP1-3 ELISA kit (HNP1-3, Human, ELISA kit, article number HK317). The epitope of HNP1-3, Human, ELISA kit is pre-coated with antibodies that recognize human HNP1-3 protein. Biotinylated antibodies will bind to captured human HNP1-3 protein, streptavidin peroxide. The enzyme label was combined with a biotin tracer and the absorbance of the reaction product at 450 nm was determined. Figure 2: Kit principle display HNP1-3, Human, ELISA kit advantages: Suitable for the detection of natural and recombinant human HNP1-3 in human serum, plasma, cell culture, and other biological fluids. The minimum detection is 156 pg/ml and the detection range is from 156 pg/ml to 10,000 pg/ml. The sample dilution buffer provided can effectively prevent the adsorption of HNP1-3 and Ig, other proteins and plastics. The entire experimental operation time is 3.5h The kit contains 12 8-well plates for more flexible experimentation The purpose and strategy of Hycult Biotech in the Netherlands is to focus on the research of natural immune-related diseases and to improve the quality of human life. As a leader in immunology and cell biology, especially in the fields of natural immunity, inflammatory response and cell damage, Hycult Biotech not only develops these products for bioscience research, but also works on these researches. The product was transformed into a clinical test study. As the general agent of many well-known brands in China and the regional agent in China, Aimei and Hycult are working together to provide researchers with the best and latest cell biology research tools to continuously expand the quality of products and services. Electronic Locks,Cylinder Locks,Stainless Steel Locks,Stainless Steel Door Lock Ningbo Hengchieh Locking Technology Co., Ltd. , https://www.hengchieh.com
